Interaction studies of two proteins labeled with Atto488 & Alexa647 by conventional TIRF and super resolution STORM microscopy in a mammalian cell
(A) Principle of N-STochastic Optical Reconstruction Microscopy [STORM](adopted from nikon webpage) (B) Total Internal Reflection(TIRF) image of the double labeled (Atto488&Alexa647) HEK-293cells.(C) Reconstructed two color STORM (super-resolution) image from: a data set of 20,000 frames visualized by N-STORM microscope.(D) Higher magnification views of the closely spaced two different protein molecules. Each localization is depicted in the STORM image as a Gaussian peak, the width of which is determined by the number of photons detected. (E) Average distance between two proteins molecules (spatial distribution) calculated by Matlab program.(F) N-STORM Super Resolution Microscope. Scale bars-10 μm (B), 100nm(C) and 50 nm (D).